Fertilization in vitro of Saimiri sciureus follicular oocytes. 1975

T J Kuehl, and W R Dukelow

An in vitro fertilization system was developed for the squirrel monkey. Oocytes recovered from follicles of gonadotropin-treated females and cultured in vitro with spermatozoa resulted in fertilized ova capable of developing to the four-cell stage in culture. In total, 264 follicular oocytes were recovered from 922 follicles in 38 Saimiri sciureus. Seventy-nine developed to the first polar body stage, and 32 of the 79 were fertilized in vitro.

UI MeSH Term Description Entries
D008297 Male Males
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D010060 Ovulation The discharge of an OVUM from a rupturing follicle in the OVARY. Ovulations
D010063 Ovum A mature haploid female germ cell extruded from the OVARY at OVULATION. Egg,Egg, Unfertilized,Ova,Eggs, Unfertilized,Unfertilized Egg,Unfertilized Eggs
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004542 Ejaculation The emission of SEMEN to the exterior, resulting from the contraction of muscles surrounding the male internal urogenital ducts. Ejaculations
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D005260 Female Females
D005306 Fertilization The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE. Conception,Fertilization, Delayed,Fertilization, Polyspermic,Conceptions,Delayed Fertilization,Delayed Fertilizations,Fertilizations,Fertilizations, Delayed,Fertilizations, Polyspermic,Polyspermic Fertilization,Polyspermic Fertilizations

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