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A human chromosomal region 11q23-specific DNA library has been constructed by means of microdissection-microcloning method with polymerase chain reaction (PCR) technique (Seki et al., Genomics 16: 1993). DNA sequences were determined for 25 microclones that contained approximately 300-500 bp insert and gave a unique (single copy) signal in Southern blot analysis. The sequence tagged site (STS) was designed and appropriate condition for PCR was determined for each unique microclone. Twelve STSs were established and used for PCR-screening of human genomic libraries constructed with yeast artificial chromosome (YAC). Thirteen YAC clones have been isolated from eight STSs. These chromosomal region-specific STSs and YAC clones will be useful in the positional cloning of disease-related genes localized to the q23 region of chromosome 11.
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
May 1995,
Cancer genetics and cytogenetics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
January 1995,
Cytogenetics and cell genetics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
October 1992,
Genomics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
May 1993,
Human molecular genetics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
June 1993,
Genomics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
September 1993,
Genomics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
November 1991,
Genomics,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
January 2004,
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
January 1995,
DNA sequence : the journal of DNA sequencing and mapping,
N Seki, and
M Yamauchi, and
T Saito, and
Y Harada, and
T Hori
January 1993,
Cytogenetics and cell genetics,
