Biomaterial Database

[Production of spheroplasts from Pseudomonas aeruginosa]. 1975

S M Trutko

The spheroplasts were prepared from the gram-negative bacterium Pseudomonas aeruginosa IBFM-13 upon incubation with lysozyme, but without EDTA. The following conditions were found to be optimal: 20 percent sucrose; lysozyme, 54 mcg/mg protein of the cells; pH 7.6. The spheroplasts, as well as the cells, have a high rate of malate oxidation, but do not oxidize NADH2, the latter fact suggesting that the cytoplasmic membrane remains intact.

UI	MeSH Term	Description	Entries
D009113	Muramidase	A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.	Lysozyme,Leftose,N-Acetylmuramide Glycanhydrolase,Glycanhydrolase, N-Acetylmuramide,N Acetylmuramide Glycanhydrolase
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D013104	Spheroplasts	Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.	Spheroplast