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We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17Leh507. Probes D17Leh502 and D17Leh507 are at the extreme ends of the YAC contig. With the exception of D17Leh507, all of these probes are within a deletion associated with the quaking(viable) (qkv) allele of quaking. We have positioned these probes on a detailed YAC physical map together with two previously published probes, D17Leh508 and D17Aus119. We show here that D17Leh508 is also within the qkv deletion. Genetic mapping of D17Leh504 and D17Leh507 on two high-resolution genetic crosses carrying qkv and quaking(lethal-1) (qkl-1) alleles shows that these probes do not recombine with quaking and are therefore within 0.04 cM of qkv and 0.05 cM of qkl-1 mutations. The deletion breakpoint contained within the YAC contig has been positioned to within 90 kb by restriction mapping of wildtype and mutant DNA. This contig will form the basis for identification and mapping of expressed sequences and for an investigation of genome organization.
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
January 1993,
European journal of human genetics : EJHG,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
July 1996,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
May 1997,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
October 1995,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
December 1995,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
January 1996,
Cytogenetics and cell genetics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
February 1994,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
June 1993,
Genomics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
September 1995,
American journal of human genetics,
R D Cox, and
A Shedlovsky, and
R Hamvas, and
M Goldsworthy, and
J Whittington, and
C S Connelly, and
W F Dove, and
H Lehrach
January 1992,
Genomics,
