Experiments were conducted on 24 dogs to elaborate two variants of precision (microsurgical) double-row suture in operations on the large intestine. The suggested variants of the suture were successfully applied in operations on the large intestine in 48 patients. The first variant of a double-row sero-serous and musculo-intramucous suture is technically simpler and preferable in formation of end to side, side to side ileotransfercal anastomoses, and endo-to end anastomoses of the large intestine. The variant of sero-serous and musculo-intramucous suture with double stitching of the submucous coat on each side of the created anastomosis is preferable in complicated restorative-re constructive interventions. Both variants (the second to a greater measure) ensure sufficient strength and air-tightness of the formed anastomoses, exact approximation of the layers of the edges of the intestine, and quicker healing of the intestinal wound by first intention. The suggested sutures make it possible to decline the formation of preventive relieving colostomas and transanal draining of large intestinal anastomoses.