-transparent.png){kind=logo}
Endoglucanase CelC from Clostridium thermocellum expressed in Escherichia coli has been crystallized in two different crystal forms by the hanging drop method. Crystals of form I were grown with polyethylene glycol as a precipitant. They are orthorhombic, space group P2(1)2(1)2(1), with cell dimensions a = 51.4 A, b = 84.3 A, and c = 87.5 A. Crystals of form II, obtained in ammonium sulfate solutions, belong to the tetragonal space group P4(1)2(1)2 (or P4(3)2(1)2) with cell dimensions of a = b = 130.7 A and c = 69.6 A. Diffraction data to 2.8 A resolution were observed for both crystal forms with a rotating anode generator. Preliminary oscillation images of the orthorhombic form I crystals using a synchrotron radiation source show diffraction to 2.2 A resolution, indicating that these crystals are suitable for high resolution crystallographic analysis.
R Dominguez, and
H Souchon, and
P M Alzari
December 1992,
Biochemical and biophysical research communications,
R Dominguez, and
H Souchon, and
P M Alzari
January 1988,
Gene,
R Dominguez, and
H Souchon, and
P M Alzari
November 1993,
Protein engineering,
R Dominguez, and
H Souchon, and
P M Alzari
October 1991,
The Biochemical journal,
R Dominguez, and
H Souchon, and
P M Alzari
April 1992,
The Biochemical journal,
R Dominguez, and
H Souchon, and
P M Alzari
March 2007,
Proceedings of the National Academy of Sciences of the United States of America,
R Dominguez, and
H Souchon, and
P M Alzari
April 2011,
Applied microbiology and biotechnology,
R Dominguez, and
H Souchon, and
P M Alzari
December 2007,
The Journal of biological chemistry,
R Dominguez, and
H Souchon, and
P M Alzari
March 1994,
Biochemistry and molecular biology international,
R Dominguez, and
H Souchon, and
P M Alzari
January 1991,
Journal of bacteriology,
