BIOMAT Database Logo BIOMAT Database Logo

Phenobarbital-induced hepatocellular proliferation: anti-bromodeoxyuridine and anti-proliferating cell nuclear antigen immunocytochemistry. 1993

H B Jones, and N A Clarke, and N C Barrass
Safety of Medicines Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom.

We report modifications to immunocytochemical detection procedures for proliferating cell nuclear antigen (PCNA) which permit its identification in liver samples previously fixed for BrdU immunocytochemistry. Both methods have been used for the assessment of phenobarbital-induced cell proliferation in rat liver. The difficulties associated with the hitherto unsuccessful application of PCNA immunocytochemical methods to tissues fixed in formalin for BrdU visualization were overcome by epitope unmasking with acid hydrolysis, extension of primary antiserum (PC10) incubation, and employment of streptavidin-ABC-HRP. BrdU delivery via osmotic minipumps for 48 hr before euthanasia, followed by fixation in cold formalin for 14 days, yielded reliable and reproducible hepatocellular labeling and a peak of cell proliferation in all lobes on Day 3 (i.e., labeling during Days 1-3) of dosing with 80 mg/kg/day phenobarbital. Labeling indices (LI) of both control and phenobarbital-treated liver were lower in the left and right median lobes as compared with the lateral lobes. In sections of the left lateral lobe from the same liver, PCNA immunocytochemistry revealed a peak of proliferative activity (about one third of the maximum LI generated by BrdU incorporation) on Day 1. These findings, together with the advantages and disadvantages of both techniques, are discussed in the context of their applications to different investigative requirements.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008938 Mitosis A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D010634 Phenobarbital A barbituric acid derivative that acts as a nonselective central nervous system depressant. It potentiates GAMMA-AMINOBUTYRIC ACID action on GABA-A RECEPTORS, and modulates chloride currents through receptor channels. It also inhibits glutamate induced depolarizations. Phenemal,Phenobarbitone,Phenylbarbital,Gardenal,Hysteps,Luminal,Phenobarbital Sodium,Phenobarbital, Monosodium Salt,Phenylethylbarbituric Acid,Acid, Phenylethylbarbituric,Monosodium Salt Phenobarbital,Sodium, Phenobarbital
D001973 Bromodeoxyuridine A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors. BUdR,BrdU,Bromouracil Deoxyriboside,Broxuridine,5-Bromo-2'-deoxyuridine,5-Bromodeoxyuridine,NSC-38297,5 Bromo 2' deoxyuridine,5 Bromodeoxyuridine,Deoxyriboside, Bromouracil
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016591 Histocytological Preparation Techniques Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope. Cytohistological Preparation Techniques,Cytohistologic Preparation Technics,Cytohistologic Preparation Technique,Cytohistologic Preparation Techniques,Cytohistological Preparation Technic,Cytohistological Preparation Technics,Cytohistological Preparation Technique,Histocytologic Preparation Technic,Histocytologic Preparation Technics,Histocytologic Preparation Technique,Histocytologic Preparation Techniques,Histocytological Preparation Technic,Histocytological Preparation Technics,Histocytological Preparation Technique,Preparation Technic, Cytohistologic,Preparation Technic, Cytohistological,Preparation Technic, Histocytologic,Preparation Technic, Histocytological,Preparation Technics, Cytohistologic,Preparation Technics, Cytohistological,Preparation Technics, Histocytologic,Preparation Technics, Histocytological,Preparation Technique, Cytohistologic,Preparation Technique, Cytohistological,Preparation Technique, Histocytologic,Preparation Technique, Histocytological,Preparation Techniques, Cytohistologic,Preparation Techniques, Cytohistological,Preparation Techniques, Histocytologic,Preparation Techniques, Histocytological,Technic, Cytohistologic Preparation,Technic, Cytohistological Preparation,Technic, Histocytologic Preparation,Technic, Histocytological Preparation,Technics, Cytohistologic Preparation,Technics, Cytohistological Preparation,Technics, Histocytologic Preparation,Technics, Histocytological Preparation,Technique, Cytohistologic Preparation,Technique, Cytohistological Preparation,Technique, Histocytologic Preparation,Technique, Histocytological Preparation,Techniques, Cytohistologic Preparation,Techniques, Cytohistological Preparation,Techniques, Histocytologic Preparation,Techniques, Histocytological Preparation,Cytohistologic Preparation Technic
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

H B Jones, and N A Clarke, and N C Barrass
Proliferation markers in the adult rodent brain: bromodeoxyuridine and proliferating cell nuclear antigen.
September 2005, Brain research. Brain research protocols,
H B Jones, and N A Clarke, and N C Barrass
Cell proliferation in rat colon measured with bromodeoxyuridine, proliferating cell nuclear antigen, and [3H]thymidine.
January 1992, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
H B Jones, and N A Clarke, and N C Barrass
Proliferating cell nuclear antigen: a marker for hepatocellular proliferation in rodents.
December 1993, Environmental health perspectives,
H B Jones, and N A Clarke, and N C Barrass
Measuring cell proliferation in the rectal mucosa. comparing bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) assays.
August 2000, Journal of clinical epidemiology,
H B Jones, and N A Clarke, and N C Barrass
Cell proliferation in prostatic adenocarcinoma: in vitro measurement by 5-bromodeoxyuridine incorporation and proliferating cell nuclear antigen expression.
February 1993, The Journal of urology,
H B Jones, and N A Clarke, and N C Barrass
Comparison of bromodeoxyuridine and proliferating cell nuclear antigen labeling in gastric carcinoma.
February 1994, Journal of Korean medical science,
H B Jones, and N A Clarke, and N C Barrass
[Anti-proliferating cell nuclear antigen (PCNA) antibody].
June 2010, Nihon rinsho. Japanese journal of clinical medicine,
H B Jones, and N A Clarke, and N C Barrass
Bromodeoxyuridine uptake and proliferating cell nuclear antigen expression throughout the colorectal tumor sequence.
January 1993, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
H B Jones, and N A Clarke, and N C Barrass
[Correlative analysis with proliferating cell nuclear antigen and bromodeoxyuridine on golden hamster carcinogenesis model]
September 1997, Shanghai kou qiang yi xue = Shanghai journal of stomatology,
H B Jones, and N A Clarke, and N C Barrass
Comparative analysis of proliferating cell nuclear antigen, bromodeoxyuridine, and mitotic index in uveal melanoma.
December 1995, Investigative ophthalmology & visual science,
Copied contents to your clipboard!