Free radicals from polycrystalline pyrimidines and purines upon heavy ion bombardment at low temperatures: an electron spin resonance study. 1993

A Schaefer, and J Hüttermann, and G Kraft
Fachrichtung Biophysik und Phys. Grundlagen der Medizin, Universität des Saarlandes, Homburg/Saar, Germany.

The formation of free radicals in polycrystalline samples of the DNA constituents thymine, cytosine, and adenosine after bombardment with heavy ions at about 100 K was investigated by electron spin resonance (ESR) spectroscopy. Spectra were observed at 77 K after irradiation at 100 K, upon annealing to 300 K and after storage at 300 K. Individual radical patterns were isolated from the spectra by computer manipulation and assigned to structures by powder-simulations based on literature data. The spectra of thymine contain an allyl radical, the octet pattern of the 5-thymyl radical and contributions of the 6-yl radical formed by net hydrogen gain at carbon C5. The latter species is also present in cytosine which in addition displays the pattern due to H-addition at the carboxyl oxygen C2. Adenosine exhibits two H-addition radicals, one at C2, the other at C8. Additionally, the spectra of all DNA subunits studied contain as a radical component a Gaussian singlet of about 0.9 mT line width. The spectra obtained at low temperature already contain the secondary radicals but exhibit a large linewidth. This feature is attributed to dipolar coupling caused by radicals in close proximity.

UI MeSH Term Description Entries
D010315 Particle Accelerators Devices which accelerate electrically charged atomic or subatomic particles, such as electrons, protons or ions, to high velocities so they have high kinetic energy. Betatrons,Linear Accelerators,Accelerator, Linear,Accelerator, Particle,Accelerators, Linear,Accelerators, Particle,Betatron,Linear Accelerator,Particle Accelerator
D003596 Cytosine A pyrimidine base that is a fundamental unit of nucleic acids.
D004578 Electron Spin Resonance Spectroscopy A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING. ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D005609 Free Radicals Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. Free radicals include reactive oxygen and nitrogen species (RONS). They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated. Free Radical
D005615 Freezing Liquids transforming into solids by the removal of heat. Melting
D000241 Adenosine A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter. Adenocard,Adenoscan
D013941 Thymine One of four constituent bases of DNA. 5-Methyluracil,5 Methyluracil

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