Repair kinetics of radiation damage in the developing rat cervical spinal cord. 1993

A C Ruifrok, and B J Kleiboer, and A J van der Kogel
Institute of Radiotherapy, University of Nijmegen, The Netherlands.

The kinetics of repair of sublethal radiation damage was examined in the cervical spinal cord of developing, 1-week-old rats. Split-dose irradiation treatments were given with time intervals of 0.5-96 h. The data, supplemented with fractionation data from previous experiments, were analysed using direct analysis based on the incomplete repair (IR) model. The best fit to the monoexponential repair model resulted in an estimated half-time of repair (T1/2) of 1.5 (1.3-1.8) h. No indications of a slow or second component of repair could be detected in the 1-week-old cervical spinal cord. This is in contrast with literature reports of experiments with the adult rat cervical spinal cord, suggesting bi-exponential repair, with 65% of the damage repaired with a slow repair T1/2 of about 4 h. Two-step methods, although statistically inferior to direct analysis, are still in use for analysis of repair experiments. A number of two-step methods used for data analysis in previous reports concerning sublethal damage repair, are dose (un)repaired, proportion of dose unrepaired, and proportion of damage unrepaired. It is argued that of the methods discussed, only analysis of the data expressing the results as proportion unrepaired damage (delta Eu) and using split-dose experiments, does not result in introduction of an artificial second repair T1/2 in tissues with a high fractionation sensitivity. Two-step analysis of the present data using delta Eu suggested monoexponential repair with a T1/2 value of 1.5 (SE 0.2) h.

UI MeSH Term Description Entries
D008297 Male Males
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004260 DNA Repair The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones. DNA Damage Response
D004307 Dose-Response Relationship, Radiation The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation. Dose Response Relationship, Radiation,Dose-Response Relationships, Radiation,Radiation Dose-Response Relationship,Radiation Dose-Response Relationships,Relationship, Radiation Dose-Response,Relationships, Radiation Dose-Response
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013116 Spinal Cord A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER. Coccygeal Cord,Conus Medullaris,Conus Terminalis,Lumbar Cord,Medulla Spinalis,Myelon,Sacral Cord,Thoracic Cord,Coccygeal Cords,Conus Medullari,Conus Terminali,Cord, Coccygeal,Cord, Lumbar,Cord, Sacral,Cord, Spinal,Cord, Thoracic,Cords, Coccygeal,Cords, Lumbar,Cords, Sacral,Cords, Spinal,Cords, Thoracic,Lumbar Cords,Medulla Spinali,Medullari, Conus,Medullaris, Conus,Myelons,Sacral Cords,Spinal Cords,Spinali, Medulla,Spinalis, Medulla,Terminali, Conus,Terminalis, Conus,Thoracic Cords
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

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