Restriction fragment length polymorphism and evolution of the mouse immunoglobulin constant region gamma loci. 1993

M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
Unité d'Immunochimie Analytique, Département d'Immunologie, Institut Pasteur (CNRS UA 359), Paris, France.

Genomic DNA from twelve laboratory mouse strains, in addition to 21 wild-derived strains belonging to different taxa (Mus musculus domesticus, Mus musculus musculus, Mus spretus, Mus macedonicus, and Mus spicilegus) and four mouse strains that are evolutionarily more distant, were analyzed by Southern blot for polymorphism of the Ig heavy chain constant region gamma isotype (Igh-C gamma) and for the distribution of the duplicated Igh-1 (C gamma 2 alpha) haplotype. Distinct allelic forms of each Igh-C locus could be defined by restriction fragment length polymorphism (RFLP). In laboratory mouse strains RFLP proved to be more sensitive in the detection of Igh-4 (C gamma 1) alleles than serological methods. Taq I digestion allowed the definition of two alleles in the Igh-8 (C gamma 3) locus, which is absolutely conserved at the protein levels. More extensive RFLP could be found in wild strains belonging to the subgenus Mus and in the evolutionarily more distant Mus species belonging to other subgenera. In previous studies we have shown that the Igh-1 locus is duplicated in M. m. musculus subspecies. We now extend this observations to the wild mouse strains belonging to M. spicilegus and M. macedonicus species and to the evolutionarily more distant wild mouse strain Mus pahari (subgenus coelomys), which is thought to have diverged from domestic mice about 5 million years ago. In addition, we found a similar RFLP pattern in ten of 18 wild mice trapped in India, suggesting that the haplotype containing the two Igh-1-like genes, organized in tandem as distinct isotypes, is widely spread in natural populations. The evolution of murine Igh-C gamma-encoded isotypes is also discussed.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007127 Immunoglobulin Constant Regions The domains of the immunoglobulin molecules that are invariable in their amino acid sequence within any class or subclass of immunoglobulin. They confer biological as well as structural functions to immunoglobulins. One each on both the light chains and the heavy chains comprises the C-terminus half of the IMMUNOGLOBULIN FAB FRAGMENT and two or three of them make up the rest of the heavy chains (all of the IMMUNOGLOBULIN FC FRAGMENT) Ig Constant Regions,Immunoglobulin Constant Region,Constant Region, Ig,Constant Region, Immunoglobulin,Constant Regions, Ig,Constant Regions, Immunoglobulin,Regions, Ig Constant
D007143 Immunoglobulin Heavy Chains The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa. Immunoglobulins, Heavy-Chain,Heavy-Chain Immunoglobulins,Ig Heavy Chains,Immunoglobulin Heavy Chain,Immunoglobulin Heavy Chain Subgroup VH-I,Immunoglobulin Heavy Chain Subgroup VH-III,Heavy Chain Immunoglobulins,Heavy Chain, Immunoglobulin,Heavy Chains, Ig,Heavy Chains, Immunoglobulin,Immunoglobulin Heavy Chain Subgroup VH I,Immunoglobulin Heavy Chain Subgroup VH III,Immunoglobulins, Heavy Chain
D008815 Mice, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation. Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D009115 Muridae A family of the order Rodentia containing 250 genera including the two genera Mus (MICE) and Rattus (RATS), from which the laboratory inbred strains are developed. The fifteen subfamilies are SIGMODONTINAE (New World mice and rats), CRICETINAE, Spalacinae, Myospalacinae, Lophiomyinae, ARVICOLINAE, Platacanthomyinae, Nesomyinae, Otomyinae, Rhizomyinae, GERBILLINAE, Dendromurinae, Cricetomyinae, MURINAE (Old World mice and rats), and Hydromyinae. Murids,Murid
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D005075 Biological Evolution The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics. Evolution, Biological
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D006239 Haplotypes The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX. Haplotype
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 1987, The Journal of clinical investigation,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
July 1996, Journal of clinical immunology,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 2002, Methods in molecular biology (Clifton, N.J.),
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 1990, Experimental and clinical immunogenetics,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
July 1990, TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 1991, TSitologiia i genetika,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
May 2001, Current protocols in human genetics,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 1985, Human genetics,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
January 1991, Annals of clinical and laboratory science,
M G Morgado, and E Jouvin-Marche, and C Gris-Liebe, and F Bonhomme, and R Anand, and G P Talwar, and P A Cazenave
August 1996, Lupus,
Copied contents to your clipboard!