Biomaterial Database

A rapid method of purification of human granulocyte cationic neutral proteases: purification and further characterization of human granulocyte elastase. 1975

G Feinstein, and A Janoff

Human granulocyte elastase (EC 3.4.21.-) was isolated and purified (yield = 62%, purity = 91-100%) by a new short procedure using affinity chromatography using phenylbutylamine covalently linked to Affi-Gel. The granulocyte elastase was found to have a molecular weight of 34 400 by sodium dodecyl sulphate gel electrophoresis and the molecular weight obtained from the amino acid composition was 34 970. The composition of elastase purified from normal leucocytes showed some significant differences from that of enzyme purified by others from leukemic leucocytes. The granulocyte elastase hydrolysed typical pancreatic elastase substrates like Boc-Ala-ONp and Ac-(Ala)3-Nan. The enzyme was also found to have a weak enzymatic activity in hydrolysing acetyl-L-phenylalanine-alpha-naphthyl ester, a typical chymotrypsin substrate. A monospecific antiserum raised against the purified enzyme gave a single precipitin line with the pure enzyme and also with crude granular extract, both lines being identical.

UI	MeSH Term	Description	Entries
D007962	Leukocytes	White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).	Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010196	Pancreatic Elastase	A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.	Elastase,Pancreatopeptidase,Elastase I,Pancreatic Elastase I,Elastase I, Pancreatic,Elastase, Pancreatic
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004589	Electrophoresis, Disc	Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.	Electrophoresis, Disk,Disc Electrophoresis,Disk Electrophoresis
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006098	Granulocytes	Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.	Granulocyte
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino