-transparent.png){kind=logo}
Restriction fragment length polymorphism (RFLP) analysis of a large number of Japanese isolates of Mycobacterium tuberculosis, containing isolates from small outbreaks of M. tuberculosis infection, and clinical isolates of M. bovis BCG, was carried out using a DNA probe derived from the insertion sequence IS986. Clinical isolates of M. tuberculosis had a high degree of RFLP. The occurrences of the IS element varied from 1 to 19, the majority of isolates having 8 to 15 copies. Very similar fingerprints, however, were seen among strains isolated in the Kanto district. In particular, 3 strains were of the same pattern with or without an additional band. Similarity of the banding patterns of strains isolated in the same district was observed in other areas. Six groups of strains, each group arising from a suspected common source of infection, were analyzed. Of these, 5 showed identical fingerprints within each group, but one showed different fingerprints. RFLP patterns of three strains isolated from individuals with lymphadenitis developed about two months after BCG vaccination, and one strain isolated from a bladder cancer patient with BCG instillation therapy were identical to those of BCG-Tokyo which had been used for the vaccination and therapy. These results confirm that RFLP analysis using IS986 is a suitable tool for epidemiology of tuberculosis.
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
January 2009,
Methods in molecular biology (Clifton, N.J.),
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
July 1994,
Journal of clinical microbiology,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
November 1995,
Thorax,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
March 1997,
Journal of clinical microbiology,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
November 2004,
Memorias do Instituto Oswaldo Cruz,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
April 2016,
Revista da Sociedade Brasileira de Medicina Tropical,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
December 1998,
Medicina clinica,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
June 2003,
Infection,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
November 2005,
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease,
M Takahashi, and
Y Kazumi, and
Y Fukasawa, and
K Hirano, and
T Mori, and
J W Dale, and
C Abe
December 2016,
International journal of mycobacteriology,
