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University of Wisconsin solution (UW) has been demonstrated to improve donor heart preservation. During reperfusion, however, myocardial performance may still remain limited by neutrophil (PMN)-mediated injury, manifested by the "low-reflow" phenomenon and myocardial "stunning." Since PMN adhesion is an important mechanism of PMN-mediated injury, we tested whether blocking PMN adhesion molecule CD18 would enhance the efficacy of UW for 15-hr heart preservation. Rabbit hearts (n = 15) were arrested with, and preserved for 15 hr in, 4 degrees C UW. After insertion of an left ventricular (LV) balloon, hearts were heterotopically transplanted into recipients pretreated with either intravenous (i.v.) saline (vehicle, n = 8) or i.v. anti-CD18 monoclonal antibody R15.7 (anti-CD18, n = 7). After 1 hr reperfusion the slope of the peak systolic pressure-volume (P-V) relation (Emax), the exponential coefficient (beta), the volume-axis intercept (Vo) of the end-diastolic P-V relation, and the time constant of the exponential LV pressure decay after dP/dt min (tau) were measured. Blood flow was measured with microspheres from which coronary vascular resistance (CVR) was calculated. Tissue %H2O was also measured. Between groups there were no significant differences in Emax (70.5 +/- 6.1 vs 63.7 +/- 6.0 mm Hg/ml, P > 0.05), beta (3.3 +/- 0.5 vs 4.3 +/- 0.5, P > 0.05), Vo (-0.4 +/- 0.1 vs -0.4 +/- 0.2 ml, P > 0.05) or %H2O (81.3 +/- 1.1 vs 80.0 +/- 0.9, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
January 1988,
The Journal of heart transplantation,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
April 1990,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
April 1990,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
October 2020,
Artificial organs,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
February 1991,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
February 1996,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
December 1993,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
February 1992,
Transplantation proceedings,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
December 1993,
The Journal of surgical research,
J G Byrne, and
M P Murphy, and
W J Smith, and
G S Couper, and
R F Appleyard, and
L H Cohn
October 2007,
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,
