BIOMAT Database Logo BIOMAT Database Logo

Identity of tumorigenic human urothelial cell lines and 'spontaneously' transformed sublines. 1993

B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Fibiger Institute, Danish Cancer Society, Copenhagen.

Restriction fragment length polymorphism (RFLP) analysis, comparative marker chromosome analysis, and polymorphic enzyme analysis was carried out on a total of eight human urothelial cell lines and sublines selected according to our knowledge of their HLA-A,B phenotype. RFLP analysis and cytogenetic analysis showed that the cell lines Hu1703He, Hu1922, and T24 are genuine cell lines of different origin. The identity of Hu1703He could not be confirmed by its isozyme phenotype which was identical to the T24 phenotype. RFLP analysis and isozyme analysis revealed that three cell lines, Hu456, Hu549, and Hu961a, and two transformed sublines, HCV-29Tmv and Hu609Tmv, are sublines of T24. A common origin of Hu456, Hu549, Hu961a, HCV-29Tmv, and Hu609Tmv was confirmed by marker chromosome analysis. However, the T24 origin of these cytogenetically related cell lines was not supported by chromosome analysis of T24. RFLP analysis and HLA phenotyping of two tumorigenic and invasive sublines isolated from a culture of non-tumorigenic Hu609 cells showed that non-tumorigenic Hu609 cells can transform 'spontaneously' in vitro into tumorigenic Hu609T cells. The results emphasise the need for careful monitoring and screening of cell lines for their identity using more than one identification parameter.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D001743 Urinary Bladder A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION. Bladder,Bladder Detrusor Muscle,Detrusor Urinae,Bladder Detrusor Muscles,Bladder, Urinary,Detrusor Muscle, Bladder,Detrusor Muscles, Bladder
D001749 Urinary Bladder Neoplasms Tumors or cancer of the URINARY BLADDER. Bladder Cancer,Bladder Neoplasms,Cancer of Bladder,Bladder Tumors,Cancer of the Bladder,Malignant Tumor of Urinary Bladder,Neoplasms, Bladder,Urinary Bladder Cancer,Bladder Cancers,Bladder Neoplasm,Bladder Tumor,Cancer, Bladder,Cancer, Urinary Bladder,Neoplasm, Bladder,Neoplasm, Urinary Bladder,Tumor, Bladder,Tumors, Bladder,Urinary Bladder Neoplasm
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002461 Cell Line, Transformed Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals. Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D002471 Cell Transformation, Neoplastic Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill. Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D005819 Genetic Markers A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event. Chromosome Markers,DNA Markers,Markers, DNA,Markers, Genetic,Genetic Marker,Marker, Genetic,Chromosome Marker,DNA Marker,Marker, Chromosome,Marker, DNA,Markers, Chromosome
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014998 Y Chromosome The male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans and in some other male-heterogametic species in which the homologue of the X chromosome has been retained. Chromosome, Y,Chromosomes, Y,Y Chromosomes

Related Publications

B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines.
August 1969, Proceedings of the National Academy of Sciences of the United States of America,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Effect of transfected interleukin-6 in non-tumorigenic and tumorigenic rat urothelial cell lines.
November 1996, International journal of cancer,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
The sialosyl Lewis a ganglioside is present in tumorigenic human urothelial cell lines.
June 1990, International journal of cancer,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Alterations of complex lipid metabolism in tumorigenic virus transformed cell lines.
January 1973, Advances in enzyme regulation,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Cell cycle perturbation by sodium butyrate in tumorigenic and non-tumorigenic human urothelial cell lines assessed by flow cytometric bromodeoxyuridine/DNA analysis.
July 1995, Cell proliferation,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Pyruvate kinase inhibited by L-cysteine as a marker of tumorigenic human urothelial cell lines.
June 1989, Experientia,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Angiogenesis-inducing ability of human bladder epithelium cell lines and "spontaneously" transformed murine fibroblasts.
December 1983, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
In vitro establishment of tumorigenic human B-lymphoblastoid cell lines transformed by Epstein-Barr virus.
November 2003, DNA and cell biology,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines.
January 1997, The Prostate,
B Christensen, and C Hansen, and M Debiec-Rychter, and J Kieler, and S Ottensen, and J Schmidt
Tumor-specific transplantation antigens in spontaneously transformed hamster cell lines.
April 1977, Cancer,
Copied contents to your clipboard!