Biomaterial Database

Analysis of L-[3H]-glutamate radioligand binding to plasma membranes of the ampullae of Lorenzini of skates. 1993

G N Akoev, and S A Dambinova, and I V Ryzhova, and A I Gorodinsky

Pavlov Institute of Physiology, Russian Academy of Sciences, St Petersburg.

Radioligand binding analysis of L-[3H]-glutamate to plasma membranes of the ampullae of Lorenzini in the skate revealed one type of binding site with KD = 286 nM and Bmax = 2.1 pmol mg-1 protein. It was revealed that Na+, K+, Ca2+ and Mg2+ inhibited the L-[3H]-glutamate binding, while Cl- had no effect. The glutamate agonists kainate, quisqualate and N-methyl-D-aspartate (NMDA) were shown to inhibit glutamate binding in a dose-dependent manner. The results obtained confirm the electrophysiological observations suggesting the existence of three types of glutamate receptor at afferent synapses of the ampullae of Lorenzini of skates.

UI	MeSH Term	Description	Entries
D007608	Kainic Acid	(2S-(2 alpha,3 beta,4 beta))-2-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid. Ascaricide obtained from the red alga Digenea simplex. It is a potent excitatory amino acid agonist at some types of excitatory amino acid receptors and has been used to discriminate among receptor types. Like many excitatory amino acid agonists it can cause neurotoxicity and has been used experimentally for that purpose.	Digenic Acid,Kainate,Acid, Digenic,Acid, Kainic
D002412	Cations	Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.	Cation
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005971	Glutamates	Derivatives of GLUTAMIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the 2-aminopentanedioic acid structure.	Glutamic Acid Derivatives,Glutamic Acids,Glutaminic Acids
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012860	Skates, Fish	The common name for all members of the Rajidae family. Skates and rays are members of the same order (Rajiformes). Skates have weak electric organs.	Raja,Rajidae,Rajiformes,Rays (Fish),Rays, Fish,Skates (Fish),Fish Ray,Fish Rays,Fish Skate,Fish Skates,Ray (Fish),Ray, Fish,Skate (Fish),Skate, Fish
D013570	Synaptic Membranes	Cell membranes associated with synapses. Both presynaptic and postsynaptic membranes are included along with their integral or tightly associated specializations for the release or reception of transmitters.	Membrane, Synaptic,Membranes, Synaptic,Synaptic Membrane
D016202	N-Methylaspartate	An amino acid that, as the D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors (RECEPTORS, NMDA).	N-Methyl-D-aspartate,NMDA,N-Methyl-D-aspartic Acid,Acid, N-Methyl-D-aspartic,N Methyl D aspartate,N Methyl D aspartic Acid,N Methylaspartate
D016318	Quisqualic Acid	An agonist at two subsets of excitatory amino acid receptors, ionotropic receptors that directly control membrane channels and metabotropic receptors that indirectly mediate calcium mobilization from intracellular stores. The compound is obtained from the seeds and fruit of Quisqualis chinensis.	Quisqualate