Biomaterial Database

Validation of the analysis of carbamazepine and its 10,11-epoxide metabolite by high-performance liquid chromatography from plasma: comparison with gas chromatography and the enzyme-multiplied immunoassay technique. 1993

J Martens, and P Banditt

Department of Clinical Pharmacology, University Hospital, Magdeburg, Germany.

An improved procedure for the determination of carbamazepine (CBZ) and its main active metabolite carbamazepine-10,11-epoxide (CBZ-e) in serum is described. The validation of this procedure shows that the limits of detection are lower than 80 ng/ml for CBZ and 7 ng/ml for CBZ-e. The relative standard deviation does not exceed 4.7% for both compounds in an inter-day precision test. In the intra-day precision test the relative standard deviations are 1.9% for CBZ-e and 1.1% for CBZ. A comparison of the described procedure with gas chromatography and the enzyme-multiplied immunoassay technique shows good agreement, but gas chromatography seems to have a relative poor reliability.

UI	MeSH Term	Description	Entries
D002220	Carbamazepine	A dibenzazepine that acts as a sodium channel blocker. It is used as an anticonvulsant for the treatment of grand mal and psychomotor or focal SEIZURES. It may also be used in the management of BIPOLAR DISORDER, and has analgesic properties.	Amizepine,Carbamazepine Acetate,Carbamazepine Anhydrous,Carbamazepine Dihydrate,Carbamazepine Hydrochloride,Carbamazepine L-Tartrate (4:1),Carbamazepine Phosphate,Carbamazepine Sulfate (2:1),Carbazepin,Epitol,Finlepsin,Neurotol,Tegretol
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D017248	Enzyme Multiplied Immunoassay Technique	An immunoenzyme test for the presence of drugs and other substances in urine and blood. The test uses enzyme linked antibodies that react only with the particular drug for which the sample is being tested.	EMIT Assay,Assay, EMIT,Assays, EMIT,EMIT Assays