Thirteen patients with Wolff-Parkinson-White syndrome were ablated with radiofrequency current. The thirteen septal atrioventricular accessory pathways in these patients included 4 right anteroseptal, 4 right posteroseptal and 5 left posteroseptal. For patients with right anteroseptal and posteroseptal accessory pathways, a large-tip ablation catheter was advanced via right femoral vein to an area just above the His bundle and around the coronary sinus ostium. For patients with left posteroseptal accessory pathways. An ablation catheter was placed at the left ventricular side of mitral annulus. All of the accessory pathways were abolished successfully. The average current applications was 14 and mean accumulated energy was 18,900 joules. The mean procedure period is 2.3 hours. There were no severe complications and there was no recurrence after 6 months of follow-up. It is shown that radiofrequency current ablation of septal accessory pathways in patients with Wolff-Parkinson-White syndrome is a safe and effective modality of treatment.