-transparent.png){kind=logo}
Growth of Neisseria gonorrhoeae in the presence of Rhesus monkey kidney (RE2) cells containing Eagle's minimal essential medium (MEM) 'conditioned' by 18-20 h of contact with RE2 cells began 3-4 h after inoculation and by 16-20 h had reached a maximum. When 'conditioned' medium (CM) was removed from the tissue cells and replaced with fresh medium a 5-6 h lag period occurred before multiplication of gonococci began and by 16 h growth was also near a maximum. CM removed from tissue cells supported the growth of six strains of N. gonorrhoeae. Three strains failed to grow in CM but multiplied when tissue cells were present. None of the strains grew in the presence of tissue cells killed by heating or in tissue-culture medium alone. Of the six strains of N. gonorrhoeae which grew in CM, three kept as their major (90%) population T1 colonies, while the remaining strains produced T1 organisms and T1A, T3, and T4 colonial variants. These results indicate that tissue cells secrete growth factors which support the growth and maintenance of T1 colonial forms of certain strains of N. gonnorhoeae.
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
October 1977,
JAMA,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
November 1978,
JACEP,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
August 1994,
Annals of the New York Academy of Sciences,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
December 1965,
Archives of ophthalmology (Chicago, Ill. : 1960),
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
December 1961,
Journal of bacteriology,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
March 1984,
Clinics in endocrinology and metabolism,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
July 1952,
Science (New York, N.Y.),
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
January 1977,
Archives of virology,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
November 1973,
Journal of theoretical biology,
F E Ashton, and
F Charron, and
F Ota, and
R Wallace, and
B B Diena
July 1952,
Science (New York, N.Y.),
