We studied the development of corticotectal synaptic terminal boutons and synapses by making injections of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) into area 17 of visual cortex in kittens ranging from newborn to 12 weeks of age and in adults. The location and extent of the injection site, and labeled corticotectal axon terminals in the superficial layers of the superior colliculus were demonstrated histochemically with the cobalt-glucose oxidase diaminobenzidine reaction. During the first 2 weeks after birth, the majority of labeled profiles resembled axonal growth cones, or structures intermediate in morphology between growth cones and synaptic terminals, while very few corticotectal axon terminals forming well-defined synaptic contacts were observed. Labeled synaptic terminals in kittens at 1 and 2 weeks of age were small, contained very few synaptic vesicles, which were usually restricted to the contact zone, and exhibited few mitochondria. By 4 and 6 weeks after birth, a well-developed population of synaptic terminals was established; however, growth cones and intermediate profiles were still numerous. At 8 weeks of age synaptic terminals were morphologically mature, and growth cone-like profiles were no longer observed. To study quantitative changes in synapse development we used the disector method to obtain unbiased estimates of the density and number of corticotectal synaptic terminals and synapses; both the density and number of terminals and synapses increased steadily throughout postnatal development. These results suggest that the corticotectal projection develops by the progressive elaboration of synapses, as opposed to synapse overproduction and subsequent elimination.