Biomaterial Database

Role of extracellular metal cations in the potential dependence of force inactivation in skeletal muscle fibres. 1993

A Schnier, and H C Lüttgau, and W Melzer

Department of Cell Physiology, Ruhr-University, Bochum, Germany.

The present experiments were designed to further characterize a metal ion binding site at the voltage sensor in the T-tubular (TT) membrane which controls the release of Ca2+ from the sarcoplasmic reticulum. For this purpose the potential dependence of force inactivation was measured under voltage clamp control in short toe muscle fibres of the frog. External solutions contained in each case one species of metal ion (Ca2+, Ba2+, Na+ and Li+, respectively). Assuming that the metal ion binds with different affinities to the resting and active state of the sensor and that the metal ion free sensor is inactivated, we estimated the dissociation constants by using the inactivation midpoint voltages (V) at different concentrations of one species of metal ion. For Ca2+ the analysis resulted in a low apparent dissociation constant KD1 (binding to the resting state) of approximately 5 x 10(-8) M and a high apparent dissociation constant KD2 > 23 mM (binding to the active state). The corresponding values for Ba2+ were: KD1 = 5 x 10(-5) M and KD2 > 125 mM. For different reasons, the data for Na+ and Li+ proved to be inconclusive.

UI	MeSH Term	Description	Entries
D008094	Lithium	An element in the alkali metals family. It has the atomic symbol Li, atomic number 3, and atomic weight [6.938; 6.997]. Salts of lithium are used in treating BIPOLAR DISORDER.	Lithium-7,Lithium 7
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D011893	Rana esculenta	An edible species of the family Ranidae, occurring in Europe and used extensively in biomedical research. Commonly referred to as "edible frog".	Pelophylax esculentus
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001464	Barium	An element of the alkaline earth group of metals. It has an atomic symbol Ba, atomic number 56, and atomic weight 138. All of its acid-soluble salts are poisonous.
D012519	Sarcoplasmic Reticulum	A network of tubules and sacs in the cytoplasm of SKELETAL MUSCLE FIBERS that assist with muscle contraction and relaxation by releasing and storing calcium ions.	Reticulum, Sarcoplasmic,Reticulums, Sarcoplasmic,Sarcoplasmic Reticulums
D012964	Sodium	A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.	Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23