Biomaterial Database

Purification and characterization of mouse serum interferon. 1975

J L Liu, and S Y Peng

Mouse serum interferon (MSIF) induced by intravenous injection with Newcastle disease virus (NDV) was subjected to purification by using Sephadex G-100 gel filtration and DEAE cellulose column chromatography. Purified IF fractions were characterized by polyacrylamide gel disc electrophoresis (PGDE) and immunodiffusion technique. Either on Sephadex G-100 gel filtration eluting with 0.1M phosphate buffer (pH 7.4) containing 0.001 M EDTA, or DEAE cellulose column chromatography eluting with 0.05 M Tris-HCl buffer (pH 8.4) containing 0.001 M EDTA and gradual increasing molarity of NaCl, two peaks were found to be associated with IF activity. The first peak from Sephadex gel filtration (I100) showed an ambiguous band located between alpha2 and beta-globulin on PGDE, while the second peak on DEAE chromatography (IID) showed no particular band on PGDE. Both the second peak which appeared on Sephadex gel filtration (II100) and the first peak appeared on DEAE chromatography (ID) presented the clear band between alpha1 and alpha2-globulin. Among these four peak fractions, ID was least contaminated by other serum protein components. Immunodiffusion analysis of whole MSIF against anti-MSIF revealed that there was only one particular band which was probably identical to II100 and ID that appeared in Ouchterlony's plate. Whole MSIF was also analysed by using cellulose acetate membrane electrophoresis (CAME). The pattern of CAME showed that the percentages of area at alpha1 and beta-globulin on the recording trace for MSIF seemed to be larger than those of control normal mouse serum.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D007369	Interferon Inducers	Agents that promote the production and release of interferons. They include mitogens, lipopolysaccharides, and the synthetic polymers Poly A-U and Poly I-C. Viruses, bacteria, and protozoa have been also known to induce interferons.	Inducers, Interferon
D007372	Interferons	Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.	Interferon
D009522	Newcastle disease virus	The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food.	Avian Paramyxovirus 1,Paramyxovirus 1, Avian
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D004589	Electrophoresis, Disc	Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.	Electrophoresis, Disk,Disc Electrophoresis,Disk Electrophoresis
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus