Regulation of undulin synthesis and gene expression in human fat-storing cells by acetaldehyde and transforming growth factor-beta 1: comparison with fibronectin. 1994

A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
Department of Clinical Pathophysiology, University of Florence, Italy.

Undulin and fibronectin (FN) are large extracellular matrix (ECM) glycoproteins possibly involved in cell-matrix interactions. In this study we analyzed the effect of acetaldehyde and transforming growth factor-beta 1 (TGF-beta 1) on undulin and FN synthesis in cultured fat-storing cells (FSC) isolated from wedge sections of normal human livers. Cultured human FSC expressed two mRNA transcripts (6.5 and 8.5 kb) specific for undulin. Acetaldehyde inhibited both undulin mRNA and protein expression, whereas it had an opposite (stimulatory) effect on FN synthesis. TGF-beta 1 induced a dose-dependent increase of both undulin and FN synthesis in FSC cultures. Furthermore, TGF-beta 1 antagonized the inhibitory effect of acetaldehyde on undulin production and potentiated the stimulatory effect of acetaldehyde on FN synthesis. Since undulin is involved in the supramolecular organization of fibrillar collagens and in their enzymatic degradation, its acetaldehyde-induced inhibition may contribute to ECM rearrangement in the early stages of alcoholic liver fibrosis.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005353 Fibronectins Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins. Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000079 Acetaldehyde A colorless, flammable liquid used in the manufacture of acetic acid, perfumes, and flavors. It is also an intermediate in the metabolism of alcohol. It has a general narcotic action and also causes irritation of mucous membranes. Large doses may cause death from respiratory paralysis. Ethanal

Related Publications

A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
July 1993, Gastroenterology,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
December 1992, Experimental cell research,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
January 1995, Hepatology (Baltimore, Md.),
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
June 1994, International journal of cancer,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
November 1995, Journal of immunology (Baltimore, Md. : 1950),
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
October 1994, Gastroenterology,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
January 1993, Virchows Archiv. B, Cell pathology including molecular pathology,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
June 2013, Biochemistry and cell biology = Biochimie et biologie cellulaire,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
November 1991, The Journal of clinical investigation,
A Casini, and E Ceni, and R Salzano, and D Schuppan, and S Milani, and G Pellegrini, and C Surrenti
December 1992, The Journal of biological chemistry,
Copied contents to your clipboard!