Biomaterial Database

Murine hepatitis caused by lymphocytic choriomeningitis virus. I. The hepatic lesions. 1994

J Löhler, and J Gossmann, and T Kratzberg, and F Lehmann-Grube

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Hamburg, Germany.

BACKGROUND The hepatitis that occurs after adult mice are infected with lymphocytic choriomeningitis (LCM) virus is immune mediated, although the details of the pathogenetic mechanisms are largely unknown. To better understand the sequence of events leading to alterations typical for hepatitides with immunopathogenesis, livers of immunocompetent mice infected with LCM virus were examined. METHODS Virus replication and histopathology in the livers and concentrations of liver enzymes in the sera of C57BL/6 mice were followed from day 3 through day 14 after intraperitoneal infection with 10(6) mouse infectious units of LCM virus. Histologic, histochemical, immunohistologic, and in situ hybridization methods were used to determine the cells involved in the inflammatory process. RESULTS Infectious virus rose to 10(9) mouse infectious units/g of liver by day 7 and declined thereafter. Viral RNA and antigen were localized in foci of hepatocytes and in Kupffer and endothelial cells of the sinusoids. Disseminated spotty necroses, steatosis, a marked sinusoidal reaction, and lobular and (later) periportal mononuclear infiltrates were observed. In the infiltrates, T cells predominated followed by macrophages and NK cells; the number of B and plasma cells rose moderately. Among T lymphocytes the CD8+ cells increased preferentially, and the CD4/CD8 ratio changed from 1.7 to 0.3. Other features were major histocompatibility complex antigen expression on hepatocytes, enhanced immunocytochemical evidence of fibronectin and ICAM-1 in sinusoids, and deposition of immunoglobulin, complement, and fibrinoid. Changed activities of liver enzymes and bilirubin levels paralleled the pathologic alterations. CONCLUSIONS Although CD8+ T cells seem to be central in the pathogenesis of LCM hepatitis, probably more than one immunopathologic mechanism is operative.

UI	MeSH Term	Description	Entries
D008216	Lymphocytic Choriomeningitis	A form of meningitis caused by LYMPHOCYTIC CHORIOMENINGITIS VIRUS. MICE and other rodents serve as the natural hosts, and infection in humans usually occurs through inhalation or ingestion of infectious particles. Clinical manifestations include an influenza-like syndrome followed by stiff neck, alterations of mentation, ATAXIA, and incontinence. Maternal infections may result in fetal malformations and injury, including neonatal HYDROCEPHALUS, aqueductal stenosis, CHORIORETINITIS, and MICROCEPHALY. (From Joynt, Clinical Neurology, 1996, Ch26, pp1-3)	Armstrong Syndrome,Armstrong's Syndrome,Encephalomyelitis, Lymphocytic Choriomeningitis Virus,Lymphocytic Choriomeningitis Virus Encephalomyelitis,Choriomeningitis, Lymphocytic,Syndrome, Armstrong,Syndrome, Armstrong's
D008217	Lymphocytic choriomeningitis virus	The type species of ARENAVIRUS, part of the Old World Arenaviruses (ARENAVIRUSES, OLD WORLD), producing a silent infection in house and laboratory mice. In humans, infection with LCMV can be inapparent, or can present with an influenza-like illness, a benign aseptic meningitis, or a severe meningoencephalomyelitis. The virus can also infect monkeys, dogs, field mice, guinea pigs, and hamsters, the latter an epidemiologically important host.	LCM Viruses,LCMV,LCM Virus,Lymphocytic choriomeningitis viruses
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D005260	Female		Females
D006524	Hepatitis, Viral, Animal	INFLAMMATION of the LIVER in animals due to viral infection.	Viral Hepatitis, Animal,Animal Viral Hepatitides,Animal Viral Hepatitis,Viral Hepatitides, Animal
D006649	Histocompatibility Antigens	A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.	Transplantation Antigens,Antigens, Transplantation,Histocompatibility Antigen,LD Antigens,SD Antigens,Antigen, Histocompatibility,Antigens, Histocompatibility,Antigens, LD,Antigens, SD
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D015870	Gene Expression	The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.	Expression, Gene,Expressions, Gene,Gene Expressions
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus