Biomaterial Database

Luteal maintenance during early pregnancy in the pig: role for prostaglandin E2. 1994

L K Christenson, and D B Farley, and L H Anderson, and S P Ford

Department of Animal Science, Iowa State University, Ames.

We previously demonstrated that prostaglandin E2 (PGE) directly inhibits prostaglandin F2 alpha (PGF)-induced regression of individual pig corpora lutea (CL) in a dose dependent manner. The present experiments were conducted to 1) characterize and compare uterine secretion of PGE and PGF during the estrous cycle and early pregnancy and 2) evaluate the local effect of the conceptus on uterine prostaglandin secretion and associated CL function in unilaterally pregnant pigs. In Experiment 1, utero-ovarian venous blood samples were collected from two nonpregnant and two pregnant gilts at 3-h intervals from day 10 through 16 (first day of estrus or mating = day 0) for quantitation of uterine PGE and PGF secretion. In Experiment 2, gilts (n = 4) were made unilaterally pregnant on day 2, and utero-ovarian venous catheters were placed bilaterally to determine if differences in PGE and/or PGF secretion might account for the known luteotrophic/antiluteolytic effect of the gravid uterine horn on the CL of the ipsilateral ovary. During the estrous cycle (Experiment 1), pulsatile secretion of PGF increased markedly on day 13 and continued to increase through day 16. PGE secretion also increased from day 13 to 16 of the estrous cycle; however, concentrations of PGE remained at least 3-fold lower than those of PGF. In contrast to changes in non-mated gilts, prostaglandin secretion in mated gilts peaked earlier (day 11-12), with PGE predominating. Thereafter, both PGE and PGF secretion declined to basal levels where they remained through day 16 of pregnancy. During unilateral pregnancy (Experiment 2), PGF concentration in nongravid and gravid horns was similar (P > 0.8). In contrast, PGE concentrations were greater (P < 0.06) in utero-ovarian venous blood draining the gravid uterine horn. This increase in PGE was associated with enhanced CL function on the ipsilateral ovary as evidenced by an elevated progesterone content and concentration as well as increased CL weights. These data are consistent with a role for conceptus-associated increases in uterine PGE secretion in the local stimulation of luteal function during early pregnancy in the pig.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008597	Menstrual Cycle	The period from onset of one menstrual bleeding (MENSTRUATION) to the next in an ovulating woman or female primate. The menstrual cycle is regulated by endocrine interactions of the HYPOTHALAMUS; the PITUITARY GLAND; the ovaries; and the genital tract. The menstrual cycle is divided by OVULATION into two phases. Based on the endocrine status of the OVARY, there is a FOLLICULAR PHASE and a LUTEAL PHASE. Based on the response in the ENDOMETRIUM, the menstrual cycle is divided into a proliferative and a secretory phase.	Endometrial Cycle,Ovarian Cycle,Cycle, Endometrial,Cycle, Menstrual,Cycle, Ovarian,Cycles, Endometrial,Cycles, Menstrual,Cycles, Ovarian,Endometrial Cycles,Menstrual Cycles,Ovarian Cycles
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011270	Pregnancy, Animal	The process of bearing developing young (EMBRYOS or FETUSES) in utero in non-human mammals, beginning from FERTILIZATION to BIRTH.	Animal Pregnancies,Animal Pregnancy,Pregnancies, Animal
D011374	Progesterone	The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.	Pregnenedione,Progesterone, (13 alpha,17 alpha)-(+-)-Isomer,Progesterone, (17 alpha)-Isomer,Progesterone, (9 beta,10 alpha)-Isomer
D011460	Prostaglandins F	(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.	PGF
D003338	Corpus Luteum	The yellow body derived from the ruptured OVARIAN FOLLICLE after OVULATION. The process of corpus luteum formation, LUTEINIZATION, is regulated by LUTEINIZING HORMONE.	Corpora Lutea,Lutea, Corpora
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog