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Thirteen X-linked, cold-sensitive lethal, female-sterile mutants of Drosophila melanogaster located at eight separate loci were screened for their ability to assemble ribosomes at the restrictive temperature of 17 degrees. Females were labelled with 3H-uridine for either 2 or 20 hours at 17 degrees. A mitochondria-free extract was prepared and analyzed by means of sucrose gradient centrifugation. Four of the mutants, l(1)TW-2cs, l(1)HM16cs, l(1)HM23cs, and l(1)HM20cs, had a lower ratio of cpm in the 40S subunit to cpm in the 60S subunit (40S:60S ratio) than wild type with a 2-hour label. The same was true of a 20-hour label of l(1)TW-2cs, l(1)HM16cs, and l(1)HM23cs, which are allelic, resulted in a 40S:60S ratio higher than wild type. Four other cs mutants were found to have less drastic effects on ribosome assembly. The ribosomal subunits of mutants l(1)HM16cs and l(1)HM20cs sediment at the same rate as their wild-type counterparts. The same is true for the RNA in their ribosomal particles. Sucrose gradient analysis of ribosomes from cold-sensitive lethal, female-sterile mutants appears to be an effective method for finding mutants that affect ribosome assembly.
E V Falke, and
T R Wright
June 1969,
Proceedings of the National Academy of Sciences of the United States of America,
E V Falke, and
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March 1974,
Genetics,
E V Falke, and
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E V Falke, and
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January 1982,
Molecular & general genetics : MGG,
E V Falke, and
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January 1981,
Genetics,
E V Falke, and
T R Wright
October 1979,
Molecular & general genetics : MGG,
E V Falke, and
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July 1981,
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E V Falke, and
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Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
E V Falke, and
T R Wright
April 1982,
Genetics,
E V Falke, and
T R Wright
November 1977,
Behavior genetics,
