The time courses of serum concentrations of prolactin, estradiol, estrone, progesterone, LH, and FSH were studied in seven pregnant rhesus monkeys from 1 month prior to delivery until 1 month after parturition. All animals nursed their young. Circulating levels of estradiol and estrone increased during the last few days of pregnancy, reaching peak values of 700 pg/ml and 350 pg/ml, respectively, on the day prior to delivery, fell precipitously to about 25 pg/ml within 1 day after parturition, and remained at this level for at least 30 days. Serum prolactin concentrations also increased during the week preceding parturition, rose abruptly at delivery, and then declined gradually. Serum progesterone levels ranged between 2 and 3 ng/ml during the last month of pregnancy, rose slightly a few days prior to parturition, decreased sharply at delivery to 50% of prepartum levels and declined gradually thereafter. Serum LH and FSH levels were not detectable during the entire sampling period. The administration of estradiol benzoate to two pregnant monkeys at midgestation, in a manner which replicated the normal prepartum increase in serum estradiol concentrations, failed to elicit an elevation in circulating prolactin levels or to induce premature delivery of the fetus.