The effect of glucose and amino acids on the synthesis of alkaline protease of the prototroph strain and auxotrophs of Bacillus subtilis A-50 was studied. There are both general and different from one another mechanisms of regulation for sporulation and protease synthesis as one of early stages of sporulation. Most auxotroph mutations pleiotropically decrease the level of protease synthesis and the efficiency of sporulation. At the same time the differences depending on sporulation and protease formation from glucose, amino acids and caseinoacids are observed. The increase in glucose concentration in the medium from 0.5 to 5% leads to intensification of protease synthesis and decrease of the sporulation efficiency. The suppression of alkaline protease formation in B. subtilis occurs when amino acid concentration is of the order of 1.5 mg/ml in the studied combination in the presence of 1--5% glucose. The strongest inhibitory effect was discovered for 2d, 3d and 4th amino acid groups in the presence of 5% glucose, whereas amino acids of the first group stimulate the protease synthesis in the presence of 2% glucose. The stimulatory effect of amino acids of the first groups is due to histidine and arginine.