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Interferon-gamma (IFN-gamma) upregulates eosinophil effector functions and prolongs the in vitro survival of eosinophils. We examined the possible capacity of IFN-gamma to stimulate eosinophils to produce eosinophil-activating cytokines. Eosinophils purified from mild atopic volunteers were cultured with 100 U/ml IFN-gamma. Viability of eosinophils was counted and supernatants were tested for the presence of cytokines by neutralization of eosinophil viability-enhancing activity with specific antibodies to IFN-gamma, interleukin-5 (IL-5), IL-3, or granulocyte-macrophage colony-stimulating factor (GM-CSF). IFN-gamma-enhanced eosinophil viability was up to 95% on the 4th day of culture. Pretreatment with anti-IL-3 antibody partially blocked the IFN-gamma-enhanced eosinophil survival. IFN-gamma-stimulated eosinophil supernatants had eosinophil survival. IFN-gamma-stimulated eosinophil supernatants had eosinophil viability-enhancing activity that was blocked by pretreatment not only with anti-IFN-gamma but also with anti-IL-3. Antibodies to IL-5 or GM-CSF did not have the blocking effect. To further confirm the production of IL-3 by eosinophils, we performed reverse transcription polymerase chain reaction (RT-PCR) for IL-3 messenger RNA (mRNA) in IFN-gamma-stimulated eosinophils. Significant IL-3 mRNA expression in eosinophils was observed at 6 h of incubation with IFN-gamma. These results suggest that IFN-gamma stimulates the autocrine function of eosinophils and may play an important role in allergic inflammation.
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
March 1986,
Journal of immunology (Baltimore, Md. : 1950),
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
January 1995,
International archives of allergy and immunology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
September 1991,
The Journal of experimental medicine,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
July 2008,
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
June 2001,
Cellular immunology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
October 2002,
Immunobiology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
December 1997,
Clinical and experimental immunology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
April 1993,
European journal of immunology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
December 1995,
The Journal of allergy and clinical immunology,
T Fujisawa, and
S Fukuda, and
J Atsuta, and
R Ichimi, and
H Kamiya, and
M Sakurai
May 1996,
Allergy,
