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The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 micrograms/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 microM and 25 microM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodo-naphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine, showed IC50 values ranging from 5 microM to 10 microM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
May 1989,
Chemical & pharmaceutical bulletin,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
February 2010,
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
December 1999,
Journal of immunological methods,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
December 2002,
Phytomedicine : international journal of phytotherapy and phytopharmacology,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
March 1999,
The Journal of pharmacy and pharmacology,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
March 1988,
Chemical & pharmaceutical bulletin,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
February 1999,
Journal of leukocyte biology,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
August 1988,
Research communications in chemical pathology and pharmacology,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
February 1995,
European journal of pharmacology,
K Ogino, and
Y Izumi, and
H Segawa, and
Y Takeyama, and
H Ishiyama, and
T Houbara, and
T Uda, and
S Yamashita
May 2009,
BMC immunology,
