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Messenger RNA sequences for immunoglobulin kappa light chain were synthesized in vitro in isolated mouse myeloma nuclei using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleoside triphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) and from isolated myeloma chromatin using exogenous Escherichia coli RNA polymerase. The in vitro RNA was transcribed using 5-mercuriuridine triphosphate and separated from in vivo RNA by chromatography on an agarose sulfhydryl affinity column. Template restriction is retained in vitro since synthesis of kappa chain messenger RNA, As determined by hybridization with complementary DNA, was much more efficient in nuclei and chromatin isolated from myeloma 66.2 tissue culture cells, a kappa-chain-producing cell line, than from MOPC 315 tissue culture cells, a lambda-chain-producing cell line. Transcription of kappa chain messenger RNA was 25 times more efficient in myeloma 66.2 nuclei than in myeloma 66.2 chromatin.
M M Smith, and
R C Huang
April 1980,
Nucleic acids research,
M M Smith, and
R C Huang
July 1979,
Nucleic acids research,
M M Smith, and
R C Huang
June 1982,
Nucleic acids research,
M M Smith, and
R C Huang
June 1980,
The Journal of biological chemistry,
M M Smith, and
R C Huang
May 1983,
Proceedings of the National Academy of Sciences of the United States of America,
M M Smith, and
R C Huang
January 1986,
Nature,
M M Smith, and
R C Huang
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M M Smith, and
R C Huang
July 1984,
The Journal of biological chemistry,
M M Smith, and
R C Huang
January 1991,
Immunogenetics,
M M Smith, and
R C Huang
October 1978,
Proceedings of the National Academy of Sciences of the United States of America,
