Biomaterial Database

The linear-plasmid-encoded toxin produced by the yeast Pichia acaciae: characterization and comparison with the toxin of Kluyveromyces lactis. 1994

D A McCracken, and V J Martin, and M J Stark, and P L Bolen

Department of Biological Sciences, Illinois State University, Normal 61790-4120.

The toxin produced by Pichia acaciae was purified and its properties compared to those of the toxin from Kluyveromyces lactis. Like this toxin, the P. acaciae toxin is a protein comprised of three subunits (molecular masses 110, 39 and 38 kDa) with an associated chitinase activity and a pH optimum between 7.0 and 7.5. P. acaciae toxin also caused G1 cell cycle arrest. Of the thirteen recessive alleles that provided resistance in Saccharomyces cerevisiae to K. lactis toxin, only three also conferred resistance to P. acaciae toxin. Similarities and differences in the interactions of the two toxins with yeast cells are discussed.

UI	MeSH Term	Description	Entries
D007716	Kluyveromyces	An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.	Kluyveromyce
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009183	Mycotoxins	Toxic compounds produced by FUNGI.	Fungal Toxins,Mycotoxin,Toxins, Fungal
D010843	Pichia	Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.	Hansenula,Hansenulas,Pichias
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002688	Chitinases	Glycoside hydrolases that break down glycosidic bonds in CHITIN. They are important for insect and worm morphogenesis and plant defense against pathogens. Human chitinases may play a role in the etiology of allergies and asthma.	Chitinase,Endochitinase
D005656	Fungal Proteins	Proteins found in any species of fungus.	Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations