BIOMAT Database Logo BIOMAT Database Logo

Bovine seminal plasma aSFP: localization of disulfide bridges and detection of three different isoelectric forms. 1994

R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Institut für Physiologie, Forschungszentrum für Milch und Lebensmittel-Weihenstephan, Freising, Germany.

Acidic seminal fluid protein (aSFP) is a major 13 kDa protein isolated from bull seminal plasma and characterized as a new growth factor which stimulates in vitro cell division and progesterone secretion by ovarian cells. Here, we establish that the four cysteines of oxidized aSFP form two disulfide bridges between nearest-neighbour residues. This pattern is conserved in boar spermadhesins, with which aSFP shares up to 50% amino acid sequence identity, and other proteins of the recently identified CUB domain family. Using isoelectric focusing in combination with sulfhydryl group-specific blotting, the three forms of aSFP were identified as completely oxidized (pI 4.7), partly reduced (pI 4.8) and fully reduced at pI 5.1. These results indicate that native aSFP possesses two pairs of cysteine residues of different reactivity. The observation that aSFP can protect sperm from oxidative damage might be explained by its reduction/oxidation behaviour.

UI MeSH Term Description Entries
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D007526 Isoelectric Point The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum. Isoelectric Points,Point, Isoelectric,Points, Isoelectric
D008297 Male Males
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010084 Oxidation-Reduction A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471). Redox,Oxidation Reduction
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003545 Cysteine A thiol-containing non-essential amino acid that is oxidized to form CYSTINE. Cysteine Hydrochloride,Half-Cystine,L-Cysteine,Zinc Cysteinate,Half Cystine,L Cysteine

Related Publications

R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Intrachain disulfide bridges of bovine seminal ribonuclease.
August 1979, Biochimica et biophysica acta,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
On multiple forms of bovine seminal plasma inhibin.
January 1991, Indian journal of biochemistry & biophysics,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Crystallization and preliminary X-ray diffraction studies of aSFP, a bovine seminal plasma protein with a single CUB domain architecture.
March 1997, Protein science : a publication of the Protein Society,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Assignment of disulfide bridges in bovine CD36.
October 1998, European journal of biochemistry,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Disulfide bridges of bovine spleen cathepsin B.
June 1990, Biological chemistry Hoppe-Seyler,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Complete primary structure of bovine beta 2-glycoprotein I: localization of the disulfide bridges.
April 1992, Biochemistry,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Isoelectric focusing of bull seminal plasma proteins.
January 1978, The International journal of biochemistry,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Localization and concentration of a new bioactive acetic seminal fluid protein (aSFP) in bulls (Bos taurus).
May 1993, Journal of reproduction and fertility,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Immunoextracted calcitonin from human seminal plasma constitutes different forms.
February 1986, Acta physiologica Scandinavica,
R Einspanier, and I Krause, and J J Calvete, and E Töfper-Petersen, and H Klostermeyer, and H Karg
Inhibition of acrosine-like protease activity by a lectin affinity chromatographic bovine seminal plasma fraction containing the PDC-109 and aSFP proteins.
September 2000, Journal of chromatography. B, Biomedical sciences and applications,
Copied contents to your clipboard!