Biomaterial Database

Neutrophil function in vitro: diapedesis and phagocytosis. 1994

E A MacDonald, and L Xia, and H Monardes, and J D Turner

Department of Animal Science, McGill University, Ste. Anne de Bellevue, PQ, Canada.

The function of neutrophils within the mammary gland was modeled in vitro to include diapedesis and phagocytosis. The bovine mammary cell line, MAC-T3, provided a mammary epithelial monolayer for use as a biologically meaningful barrier to neutrophil diapedesis. Features included characteristic transepithelial resistance, tight junctional complexes, and polarity. Continuous readings of transepithelial resistance indicated a stable resistance over several hours. Staphylococcus aureus, at concentrations of 1 x 10(7) and 2 x 10(9) cfu/ml, did not appear to have any deleterious effects on monolayer integrity over short-term (1 to 2 h) exposure. Neither resting nor challenged neutrophils caused short-term damage to the monolayer. Transepithelial resistance of the monolayers remained unchanged even as neutrophils were actively migrating through the monolayer. Further work using the MAC-T3 cell line and electrical resistance to assess cell monolayer integrity could provide much insight into the mechanisms underlying degeneration of mammary epithelial cells. The ability of neutrophils to phagocytose foreign particles is important for protection of the mammary gland. Neutrophils from proven bulls varied in their rate and capacity of phagocytosis. Correlations between neutrophil function and production traits were negative and small. In vitro analysis of neutrophil function provides another tool for the study of natural mastitis resistance.

UI	MeSH Term	Description	Entries
D007365	Intercellular Junctions	Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)	Cell Junctions,Cell Junction,Intercellular Junction,Junction, Cell,Junction, Intercellular,Junctions, Cell,Junctions, Intercellular
D008297	Male		Males
D008321	Mammary Glands, Animal	MAMMARY GLANDS in the non-human MAMMALS.	Mammae,Udder,Animal Mammary Glands,Animal Mammary Gland,Mammary Gland, Animal,Udders
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell

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