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Apoptosis is induced in immature thymocytes by physiological peak levels of glucocorticoid hormones, especially in murine and rat cells. Endogenous glucocorticoids may have some role in thymic selection. Glucocorticoid-induced thymocyte apoptosis appears to be dependent on protein kinase C (PKC), since it is inhibited by PKC inhibitors. PKC is a family of closely related enzymes, consisting of Ca(2+)-dependent (PKC-alpha, -beta I, -beta II, and -gamma) and Ca(2+)-independent (PKC-delta, -epsilon, -eta (L), -theta, -zeta, and -lambda) isozymes. In the present study, we analyzed the role of PKC in glucocorticoid-induced apoptosis in murine thymocytes and found that glucocorticoid selectively induces an increase in Ca(2+)-independent PKC activity in the particulate fraction of immature thymocytes but not in that of mature T cells. The increase as well as the apoptosis was inhibited by actinomycin D, cycloheximide, or the glucocorticoid receptor antagonist, RU 38486. Immunoblotting studies revealed the selective translocation of PKC-epsilon from the cytosolic fraction to the particulate fraction upon glucocorticoid treatment. These results suggest that glucocorticoid-induced apoptosis in immature thymocytes involves glucocorticoid receptor-mediated and selective activation of PKC-epsilon through de novo synthesis of macromolecules.
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
September 2000,
Biochimica et biophysica acta,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
January 2004,
Endocrinology,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
September 1995,
General pharmacology,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
February 1990,
Cellular immunology,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
May 1995,
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
January 2003,
Journal of cellular and molecular medicine,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
September 2001,
Cell death and differentiation,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
January 2003,
Cell biology international,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
May 2004,
The Journal of pharmacology and experimental therapeutics,
M Iwata, and
R Iseki, and
K Sato, and
Y Tozawa, and
Y Ohoka
September 2000,
Experimental cell research,
