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We have studied the effect of site-specific cytosine methylation on the binding of nuclear proteins to the gamma-globin promoter region from -71 to -34. This sequence was recently shown to contain a stage selector element responsible for increased transcription from the gamma compared to the beta promoter when both are linked to Locus Control Region enhancer sequences. We observed that Sp1 binding to this sequence is increased upon site-specific cytosine methylation, such that only methylation of the cytosine in the -50 CpG dinucleotide effects Sp1 binding. Methylation of the distal C of the Hpall site has no effect. The possible functional role of Sp1 as a repressor of gamma-globin synthesis is discussed.
P K Sengupta, and
D Lavelle, and
J DeSimone
August 1987,
The EMBO journal,
P K Sengupta, and
D Lavelle, and
J DeSimone
June 1993,
Molecular and cellular biology,
P K Sengupta, and
D Lavelle, and
J DeSimone
December 1989,
Nucleic acids research,
P K Sengupta, and
D Lavelle, and
J DeSimone
September 2000,
The Biochemical journal,
P K Sengupta, and
D Lavelle, and
J DeSimone
January 1990,
Molecular and cellular biology,
P K Sengupta, and
D Lavelle, and
J DeSimone
July 1999,
Oncogene,
P K Sengupta, and
D Lavelle, and
J DeSimone
January 1989,
Progress in clinical and biological research,
P K Sengupta, and
D Lavelle, and
J DeSimone
April 1988,
Proceedings of the National Academy of Sciences of the United States of America,
P K Sengupta, and
D Lavelle, and
J DeSimone
October 1991,
Blood,
P K Sengupta, and
D Lavelle, and
J DeSimone
October 2019,
Cell death & disease,
