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The aim of this study was to investigate the effect of various methodological procedures or protocols on cyclic AMP formation in human leucocytes. The data showed that: (1) ATP content and lactate production was unaffected by hypotonic lysis during leucocyte isolation; (2) there was a linear relation between cell number/sample and the production of cyclic adenosine 3':5'-monophosphate (cAMP); (3) the interindividual variation markedly affected cAMP production during long observation periods (years), whereas day-to-day variation within a week was less important; (4) whole blood could be stored for up to 4 h (at 4 degrees C or 23 degrees C) without affecting cAMP accumulation; (5) isolated MNL could be stored for up to 2 h (at 4 degrees C) without affecting cAMP accumulation; and finally that (6) choice and concentration of phosphodiesterase inhibitors markedly influenced the basal and isoprenaline-induced cAMP accumulation. Evidence is provided that the rate of cAMP accumulation in response to isoprenaline of cells incubated in the presence of 2.5 mM 3-isobutyl-1-methyl-xanthine (IBMX) is reflecting the rate of cellular cAMP production.
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
January 1990,
British journal of clinical pharmacology,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
June 1981,
Acta endocrinologica,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
May 1987,
General and comparative endocrinology,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
September 1985,
Acta pharmacologica et toxicologica,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
December 1976,
Biochemical pharmacology,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
October 1986,
Atherosclerosis,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
August 1981,
Endocrinologia japonica,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
February 1978,
The Journal of endocrinology,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
February 1995,
Biochemical Society transactions,
T Johansen, and
U G Friis, and
A K Rasmussen, and
N Foged
July 1973,
Endocrinology,
