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Sequences coding for the cytoplasmic and transmembrane domains were removed from the cDNA of the human Golgi resident membrane protein beta 1,4 galactosyltransferase (gal-T). The remaining sequences coding for the stem and catalytical domains of this glycosyltransferase were fused to sequences coding for the yeast invertase signal sequence. The hybrid was inserted together with a constitutive yeast promoter and a terminator into a E. coli/yeast shuttle vector. Saccharomyces cerevisiae strain BT150 transformed with this new expression vector expressed enzymically active soluble enzyme, whereas no activity was detectable in mock-transformed yeasts. The enzyme product was identified by HPLC analysis and shown to correspond to the expected product N-acetyllactosamine.
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
May 1991,
Biochemical and biophysical research communications,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
August 1994,
Gene,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
July 1996,
European journal of biochemistry,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
July 1997,
International journal of oncology,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
February 1995,
Nihon rinsho. Japanese journal of clinical medicine,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
August 2001,
Journal of cancer research and clinical oncology,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
September 2009,
Journal of cellular biochemistry,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
April 2007,
Inflammation,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
January 1998,
Proceedings of the National Academy of Sciences of the United States of America,
R Kleene, and
C H Krezdorn, and
G Watzele, and
B Meyhack, and
G F Herrmann, and
C Wandrey, and
E G Berger
August 1999,
Pancreas,
