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In order to take advantage of non-radioactive methods, we have developed two plasmids (p lambda LE and p lambda RE) for mapping restriction sites of long inserts cloned in phage lambda vectors. These plasmids are constructed by cloning the left 402-bp and right 560-bp phage lambda genome ends, respectively. To map restriction sites, the cloned sequences in p lambda LE and p lambda RE are labeled with digoxygenin and hybridized to partially digested lambda DNA. The ladder of bands detected with these probes can be used to construct restriction maps in the same way as those obtained using radioactively labeled cos complementary oligodeoxyribonucleotides [Rackwitz et al., Gene 30 (1984) 195-200].
J Piñol, and
O Cabré
October 1984,
Gene,
J Piñol, and
O Cabré
January 1990,
Yi chuan xue bao = Acta genetica Sinica,
J Piñol, and
O Cabré
March 1995,
BioTechniques,
J Piñol, and
O Cabré
July 1991,
Gene,
J Piñol, and
O Cabré
May 2001,
Current protocols in molecular biology,
J Piñol, and
O Cabré
December 1993,
Gene,
J Piñol, and
O Cabré
January 1976,
Doklady Akademii nauk SSSR,
J Piñol, and
O Cabré
April 1995,
Nature genetics,
J Piñol, and
O Cabré
January 1976,
Doklady Akademii nauk SSSR,
