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Genes homologous to those encoding mitochondrial NADH dehydrogenase subunits ND4L and ND5 in filamentous fungi were identified in the mitochondrial genome of a budding yeast, Hansenula wingei. The structure and expression of these genes were investigated. The H. wingei ND4L gene is 282 bp long, and potentially codes for a polypeptide of 94 amino acids. The putative ND4L protein sequence shares about 46% homology with the analogous mitochondrial proteins of filamentous fungi. The H. wingei ND5 gene is 1935 bp long, and encodes a 645-residue polypeptide. The derived ND5 protein shares about 38% sequence homology with the analogue in filamentous fungi. The ND4L and ND5 genes have no intervening sequence, and form a gene cluster in the order of 5'-ND4L-ND5-3'. A presumptive mature dicistronic or polycistronic transcript of these genes was detected by Northern blot hybridization. These results strongly indicate that these ND4L and ND5 genes are active. As far as we are aware, this is the first report on the identification of mitochondrially encoded ND genes in yeast.
K Okamoto, and
T Sekito, and
K Yoshida
May 1992,
Nucleic acids research,
K Okamoto, and
T Sekito, and
K Yoshida
March 1963,
Journal of bacteriology,
K Okamoto, and
T Sekito, and
K Yoshida
August 2010,
FEMS yeast research,
K Okamoto, and
T Sekito, and
K Yoshida
September 1992,
Current genetics,
K Okamoto, and
T Sekito, and
K Yoshida
September 1994,
Journal of bacteriology,
K Okamoto, and
T Sekito, and
K Yoshida
September 1968,
Bacteriological reviews,
K Okamoto, and
T Sekito, and
K Yoshida
August 1984,
Current genetics,
K Okamoto, and
T Sekito, and
K Yoshida
March 2003,
Mitochondrion,
K Okamoto, and
T Sekito, and
K Yoshida
November 1961,
Journal of general microbiology,
K Okamoto, and
T Sekito, and
K Yoshida
May 2013,
The American journal of tropical medicine and hygiene,
