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A TSH radioimmunoassay is described, which was used to investigate the behaviour of the TSH pituitary response (as measured by the secretory area) to TRH in groups of subjects of both sexes and different phases of gonadal activity. It is concluded that the TSH pituitary response is in no way affected by physiological variations in the plasma gonadal hormones. This independent behaviour of the TSH response, uninfluenced by gonadal activity, was apparently confirmed by the results in some pathological conditions (ovarian agenesis and primary male hypogonadism of various types) characterized by very low plasma levels of gonadal hormones.
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