Biomaterial Database

Comparison of counterimmunoelectrophoresis (CIE) with other serologic tests in the detection of antibodies to Trypanosoma cruzi. 1976

F G Aguilar-Torres, and M W Rytel, and I G Kagan

Application of counterimmunoelectrophoresis (CIE) in detection of antibodies against Trypanosoma cruzi in sera of 180 subjects from an endemic area (Bolivia), and 50 subjects from a nonendemic area (U.S.A.) was carried out, and the results were compared to ones obtained with latex agglutination (LA) and indirect hemagglutination (IHA). The optimal conditions for CIE were determined to be as follows: barbitone buffer of pH 8.2 and ionic strength 0.05 M was used in the chambers and in preparing the 1% agarose plates. The wells in the plates were 5 mm in diameter and 3 mm apart. Electric current was applied at 500 volts and 25 mA for 60 minutes. T. cruzi antigen (Center for Disease Control) was used in a dilution of 1:2. The sera were studied undiluted. The study population comprised the following groups: LA+ IHA+ (group I); LA+ IHA- (group II); LA- IHA+ (group III); LA- IHA- (group IV); and the control group (group V). Each group was comprised of 50 sera, except for group II which was comprised of only 30 sera. Antibodies were detected by CIE in 94% of sera in group I, 40% in group II, 76% in group III and 6% in group IV. There were no positive reactions in group V. CIE was positive in 74% of sera from the combined LA+ serum groups (I and II), and in 85% of sera from the IHA+ groups (I and III). A good correlation between CIE and the other tests in the LA+ and IHA+ group (I), and the lack of false positive reactions in group V suggest that CIE is a good diagnostic method for detection of T. cruzi antibodies. However, this study showed that IHA is a more sensitive test in epidemiological surveys than CIE. The finding of 6% sera positive by CIE in group IV may indicate a lower specificity of the test.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D007841	Latex Fixation Tests	Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)	Latex Agglutination Tests,Agglutination Test, Latex,Agglutination Tests, Latex,Fixation Test, Latex,Fixation Tests, Latex,Latex Agglutination Test,Latex Fixation Test,Test, Latex Agglutination,Test, Latex Fixation,Tests, Latex Agglutination,Tests, Latex Fixation
D003378	Counterimmunoelectrophoresis	Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.	Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D006386	Hemagglutination Tests	Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)	Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D014349	Trypanosoma cruzi	The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.	Trypanosoma cruzus,cruzi, Trypanosoma
D014355	Chagas Disease	Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.	Trypanosomiasis, South American,American Trypanosomiasis,Chagas' Disease,Trypanosoma cruzi Infection,Infection, Trypanosoma cruzi,Infections, Trypanosoma cruzi,South American Trypanosomiasis,Trypanosoma cruzi Infections,Trypanosomiasis, American