Biomaterial Database

Effect of low temperature storage on the haemolytic complement of cattle and sheep. 1993

V S Pandey, and A Verhulst, and F Demey

Institute of Tropical Medicine, Antwerp, Belgium.

Sera from cattle and sheep stored at -70, -20 and -10 degrees C were examined for haemolytic complement at Days 0, 37, 54, 86, 133 and 245. The decline in complement followed a similar trend, both by classical pathway (CPW) and alternative pathway (APW) assay methods. Both cattle and sheep sera retained 80% of their complement activity till Day 245 at -70 degrees C whereas at -20 degrees C cattle sera had lost 67% and sheep sera 80% of the activity. At -10 degrees C the decline in complement levels was, in general, similar to -20 degrees C. The complement level of sheep sera declined more rapidly than that of cattle. For reliable results, it is suggested that sera of cattle and sheep should be assayed for complement within 4-5 months at -70 degrees C and within a month at -20 or -10 degrees C.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D001793	Blood Preservation	The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).	Blood Preservations,Preservation, Blood,Preservations, Blood
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003165	Complement System Proteins	Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).	Complement Proteins,Complement,Complement Protein,Hemolytic Complement,Complement, Hemolytic,Protein, Complement,Proteins, Complement,Proteins, Complement System
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D015925	Cryopreservation	Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.	Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic
D015941	Complement Hemolytic Activity Assay	A screening assay for circulating COMPLEMENT PROTEINS. Diluted SERUM samples are added to antibody-coated ERYTHROCYTES and the percentage of cell lysis is measured. The values are expressed by the so called CH50, in HEMOLYTIC COMPLEMENT units per milliliter, which is the dilution of serum required to lyse 50 percent of the erythrocytes in the assay.	CH50 Assay,Complement H50,Hemolytic Titration Assay,Serum Complement Titer,Total Hemolytic Complement,Whole Complement Titer,CH(50),Assay, CH50,Assay, Hemolytic Titration,Assays, CH50,Assays, Hemolytic Titration,CH50 Assays,Complement H50s,Complement, Total Hemolytic,Complements, Total Hemolytic,Hemolytic Complement, Total,Hemolytic Complements, Total,Hemolytic Titration Assays,Serum Complement Titers,Titer, Serum Complement,Titer, Whole Complement,Titers, Serum Complement,Titers, Whole Complement,Titration Assay, Hemolytic,Titration Assays, Hemolytic,Total Hemolytic Complements,Whole Complement Titers