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Human neutrophil procollagenase was activated by incubation with recombinant active stromelysin. Activation was achieved by cleavage of the Gly78-Phe79 peptide bond at the end of the propeptide domain in a single-step activation mechanism. In addition, accelerated activation was achieved when N-terminally truncated, latent collagenase (with Phe49 as its N-terminal residue) was incubated with recombinant active stromelysin. Determination of the specific activity of recombinant-stromelysin-activated neutrophil collagenase with dinitrophenyl-octapeptide or type I collagen demonstrated the generation of high specific activity. The specific activity of stromelysin-activated enzyme was considerably higher than that of trypsin- or HgCl2-activated collagenase. Thus human neutrophil collagenase is superactivated, like the homologous fibroblast collagenase [Murphy, Cockett, Stephens, Smith and Docherty (1987) Biochem. J. 248, 265-268]. The occurrence of Phe79 at the N-terminus of the neutrophil collagenase seemed to be critical for superactivation, which is in agreement with data published by Suzuki, Enghild, Morodomi, Salvesen and Nagase [(1990) Biochemistry 29, 10261-10270] on fibroblast collagenase.
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
January 1996,
European journal of biochemistry,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
September 1994,
Annals of the New York Academy of Sciences,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
November 1990,
Biochemistry,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
June 1997,
Archives of biochemistry and biophysics,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
November 1987,
The Biochemical journal,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
January 1989,
Reviews of infectious diseases,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
February 1996,
Arthritis and rheumatism,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
July 1988,
Collagen and related research,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
July 1994,
The Journal of biological chemistry,
V Knäuper, and
S M Wilhelm, and
P K Seperack, and
Y A DeClerck, and
K E Langley, and
A Osthues, and
H Tschesche
December 1991,
European journal of biochemistry,
