Biomaterial Database

Typing of Aeromonas spp. by numerical analysis of immunoblotted SDS-PAGE gels. 1993

R Mulla, and S Millership

Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London.

One hundred and three isolates of Aeromonas spp., collected from both environmental sources and patients, were examined by SDS-PAGE of whole cells followed by immunoblotting with polyclonal rabbit antiserum raised against whole cells of A. sobria. All isolates were typable, yielding 15-20 well separated bands. Reproducibility of the technique was good and discrimination excellent, yielding 30 types amongst 103 isolates. Immunoblot type was not related to biochemical phenotype. Attempts to correlate immunoblot type with serotype were unsuccessful because only 42% of the strains tested could be serotyped.

UI	MeSH Term	Description	Entries
D007223	Infant	A child between 1 and 23 months of age.	Infants
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004783	Environmental Microbiology	The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.	Microbiology, Environmental
D005260	Female		Females
D005516	Food Microbiology	The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.	Microbiology, Food
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man