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A dot enzyme linked immunosorbent assay (dot-ELISA) was previously developed to detect specific antibodies in rabbits sera immunized against F1A protein obtained from Yersinia pestis. This antigen was covalently linked onto the surface of dacron (polyethyleneterephthalate). Here, standard conditions are described for the optimization of this procedure: an amount of 20 ng of F1A protein was fixed onto dacron; anti-rabbit IgG peroxidase conjugate diluted 1:8,000 and 30% non-fat instant milk as blocking substance were used throughout the method. This procedure was compared with that employing nitrocellulose as solid-phase which showed to be more sensitive. However, the method based on dacron did not show false positive reactions against non-immunized rabbits sera at low antigen amount and diluted anti-IgG peroxidase conjugate.
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
November 1984,
The American journal of tropical medicine and hygiene,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
December 1989,
Journal of the Egyptian Society of Parasitology,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
October 1988,
Laboratory animal science,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
August 1989,
The Journal of parasitology,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
January 1991,
Revista do Instituto de Medicina Tropical de Sao Paulo,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
January 1997,
Revista do Instituto de Medicina Tropical de Sao Paulo,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
August 1988,
Annals of tropical medicine and parasitology,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
March 1999,
Contemporary topics in laboratory animal science,
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
January 1994,
Methods in molecular biology (Clifton, N.J.),
S M Montenegro, and
A M De Almeida, and
L B Carvalho Júnior
December 1978,
Hospital practice,
