-transparent.png){kind=logo}
New strategies for oligonucleotide synthesis by use of 2-trimethylsilylethyl and 2-trimethylsilylethoxymethyl as the phosphate and 2'-hydroxyl protecting groups, respectively. 1993
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
The 2-Trimethylsilylethyl (TSE) group was found to be useful as a protecting group for the internucleotidic phosphate in DNA synthesis. Oligodeoxyribonucleotides were synthesized in good yields by using phosphoramidite building blocks having the TSE group. In RNA synthesis, 2-trimethylsilylethoxymethyl (SEM) group was introduced as a new protecting group for the 2'-hydroxyl. Selective protection of this function with SEM-Cl was achieved in good yield by use of 3',5'-O-bis(tert-butyl)silanediyl ribonucleoside derivatives.
Related Publications
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
August 1986,
Nucleic acids research,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
February 2002,
Carbohydrate research,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
January 2005,
Nucleic acids symposium series (2004),
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
January 1994,
Methods in molecular biology (Clifton, N.J.),
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
January 2005,
Nucleosides, nucleotides & nucleic acids,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
December 1995,
Nucleic acids research,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
February 2001,
Journal of the American Chemical Society,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
March 1973,
The Journal of organic chemistry,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
March 1976,
Nucleic acids research,
T Wada, and
M Tobe, and
T Nagayama, and
K Furusawa, and
M Sekine
March 2017,
Current protocols in nucleic acid chemistry,
