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A potent and selective P type calcium channel blocker isolated from the venom of the funnel web spider Agelenopsis aperta, omega-agatoxin IVA, and its related peptides were synthesized by the solution procedure. Synthetic omega-agatoxin IVA was found to block high-threshold P-type calcium current in rat Purkinje neuron with the same potency as that reported for the natural product. Its disulfide structure was determined by amino acid analysis, gas-phase sequencing and mass spectrometry of the proteolytic fragments. The N-terminus biotinylated and truncated peptides showed the same disulfide-bond-forming profile and the same activities as those of omega-agatoxin IVA, indicating that the N-terminal basic tripeptide, Lys-Lys-Lys, is not important for both the folding and the expression of the biological activity. However, the Trp residue in the molecule might be essential for the toxin to bind tightly with the channel pores.
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
August 1994,
European journal of pharmacology,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
August 1996,
Neuroscience letters,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
January 1996,
Journal of peptide science : an official publication of the European Peptide Society,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
October 1996,
European journal of pharmacology,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
December 1993,
FEBS letters,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
March 1995,
FEBS letters,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
July 1995,
Journal of neuroscience research,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
September 1996,
European journal of pharmacology,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
April 2004,
Neurochemical research,
H Nishio, and
K Y Kumagaye, and
S Kubo, and
Y N Chen, and
A Momiyama, and
T Takahashi, and
T Kimura, and
S Sakakibara
January 1999,
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
