Biomaterial Database

Thermal inactivation of pantothenase from Pseudomonas fluorescens. 1976

R K Airas

Protection of pantothenase (pantothenate amidohydrolase, EC 3.5.1.22) against thermal inactivation by ligands was studied. The protective properties of 162 different small-molecule compounds were tested to find the most effective compounds. The best protective results were obtained with oxamate, 3-oxoglutarate, 2-oxomalonate, oxalate and oxaloacetate, in that order. The protection constants (eta) of certain metabolic intermediates were estimated using differential thermal inactivation. Generally they were observed to be lower than the corresponding inhibition constants. The deltaH of the oxalate binding derived from eta values was --165 kJ/mol. Normally, the activation energy of thermal inactivation of pantothenase in the absence of protective compounds is 220 kJ/mol, and the protective ligands enhance the measured activation energies of inactivation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010205	Pantothenic Acid	A butyryl-beta-alanine that can also be viewed as pantoic acid complexed with BETA ALANINE. It is incorporated into COENZYME A and protects cells against peroxidative damage by increasing the level of GLUTATHIONE.	Vitamin B 5,Calcium Pantothenate,Dexol,Vitamin B5,Zinc Pantothenate,B 5, Vitamin,B5, Vitamin,Pantothenate, Calcium,Pantothenate, Zinc
D011489	Protein Denaturation	Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.	Denaturation, Protein,Denaturations, Protein,Protein Denaturations
D011551	Pseudomonas fluorescens	A species of nonpathogenic fluorescent bacteria found in feces, sewage, soil, and water, and which liquefy gelatin.	Bacillus fluorescens,Bacillus fluorescens liquefaciens,Bacterium fluorescens,Liquidomonas fluorescens
D002151	Calorimetry	The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D002264	Carboxylic Acids	Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.	Carboxylic Acid,Acid, Carboxylic,Acids, Carboxylic
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000581	Amidohydrolases	Any member of the class of enzymes that catalyze the cleavage of amide bonds and result in the addition of water to the resulting molecules.	Amidases,Amidohydrolase
D013816	Thermodynamics	A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)	Thermodynamic