Biomaterial Database

[Introduction of the bacteriophage Mu into a nitrogen-fixing strain Klebsiella pneumoniae M 5 a 1]. 1976

C Rosenberg, and J Dénarié

Mu cts 62 a thermo-inductible mutant of phage Mu was integrated in E. coli in to the broad host range RP4 plasmid. The hybrid plasmid RP4::Mu cts 62 was then transferred by mating to the dinitrogen-fixing strain K. pneumoniae M5al. In Klebsiella Mu cts 62 is still heat inducible and the phage production is similar to that observed in E. coli. Mu Should be a useful tool for the genetic analysis of nitrogen fixation.

UI	MeSH Term	Description	Entries
D007711	Klebsiella pneumoniae	Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.	Bacillus pneumoniae,Bacterium pneumoniae crouposae,Hyalococcus pneumoniae,Klebsiella pneumoniae aerogenes,Klebsiella rhinoscleromatis
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D009586	Nitrogen Fixation	The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.	Diazotrophy,Diazotrophic Activity,Dinitrogen Fixation,N2 Fixation,Activities, Diazotrophic,Activity, Diazotrophic,Diazotrophic Activities,Fixation, Dinitrogen,Fixation, N2,Fixation, Nitrogen
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D005818	Genetic Engineering	Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.	Genetic Intervention,Engineering, Genetic,Intervention, Genetic,Genetic Interventions,Interventions, Genetic
D001435	Bacteriophages	Viruses whose hosts are bacterial cells.	Phages,Bacteriophage,Phage
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications