Biomaterial Database

Crystallographic refinement of Bowman-Birk type protease inhibitor A-II from peanut (Arachis hypogaea) at 2.3 A resolution. 1993

A Suzuki, and T Yamane, and T Ashida, and S Norioka, and S Hara, and T Ikenazka

Department of Biotechnology, Faculty of Engineering Nagoya University, Japan.

The crystal structure of Bowman-Birk type protease inhibitor A-II from peanut was refined at 2.3 A resolution using a restrained least-squares method. The crystallographic R-factor is 0.196 for 7697 reflections with F > 3 sigma (F) in the range from 6.0 to 2.3 A resolution. Two molecules in an asymmetric unit are independently refined and, their structures are compared with each other. The inhibitor molecule has an elongated shape with two reactive sites, one at both ends of the longest dimension. As a secondary structure, a 4-stranded beta-sheet-like structure is found, in which two water molecules bind two 2-stranded beta-sheets together with six hydrogen bonds. The molecule is constructed by two homologous domains which are related by an intramolecular pseudo 2-fold axis. The structure and atomic B-factors of peptide loops containing a reactive site were compared with that of adzuki bean Bowman-Birk type inhibitor in the complex with bovine beta-trypsin. This comparison shows that no significant structural change occurs in the reactive site of inhibitor at the formation of the inhibitor-protease complex, but structural rigidity around the reactive site seems to increase.

UI	MeSH Term	Description	Entries
D008958	Models, Molecular	Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.	Molecular Models,Model, Molecular,Molecular Model
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010367	Arachis	A plant genus of the family FABACEAE that includes peanuts.	Arachis hypogaea,Peanuts,Peanut
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D006860	Hydrogen Bonding	A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.	Hydrogen Bonds,Bond, Hydrogen,Hydrogen Bond
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012639	Seeds	The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.	Diaspores,Elaiosomes,Embryos, Plant,Plant Embryos,Plant Zygotes,Zygotes, Plant,Diaspore,Elaiosome,Embryo, Plant,Plant Embryo,Plant Zygote,Seed,Zygote, Plant
D013816	Thermodynamics	A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)	Thermodynamic
D014358	Trypsin Inhibitor, Bowman-Birk Soybean	A low-molecular-weight protein (minimum molecular weight 8000) which has the ability to inhibit trypsin as well as chymotrypsin at independent binding sites. It is characterized by a high cystine content and the absence of glycine.	Bowman-Birk Soybean Trypsin Inhibitor,Trypsin Inhibitor, Bowman Birk Soybean,Bowman Birk Soybean Trypsin Inhibitor
D014361	Trypsin Inhibitors	Serine proteinase inhibitors which inhibit trypsin. They may be endogenous or exogenous compounds.	Trypsin Inhibitor,Inhibitor, Trypsin,Inhibitors, Trypsin